Fig. 3
Muguga derived Tp1214–224 tetramer staining of PBMC from the three A18+ animals. BE017, BE033, BE043 and the control animal (BE029), isolated at day 15 after immunization. PBMC were co-stained with anti-CD8 Ab-PerCP and Tp1214–224-tetramer-PE. Initial gating was performed on the live lymphocyte fraction (not shown). Staining of the CD8+ cells by the tetramer is seen in the upper right quadrant (Q2). The animals from which the CD8+ cells were derived are indicated in the figure. The experiment was performed twice