Fig. 1
Effect of Z-Gly-RGD(DPhe)-mitP on HaCaT keratinocyte mitochondrial activity. a Cells were treated with Z-Gly-RGD(DPhe)-mitP for 24 h prior to evaluation of cell viability by MTT assay. b The proportions of cells depolarised after Z-Gly-RGD(DPhe)-mitP treatment, assessed by JC-1 staining and normalised to untreated cells. Data were pooled from three independent experiments performed in duplicate. c Representative data showing increase of cell population with mitochondrial depolarisation (P11) indicated by JC-1 staining after 24 h treatment with the indicated concentrations of Z-Gly-RGD(DPhe)-mitP. Data in shown in (a) and (b) are means + SEM; *p < 0.05, **p < 0.01, p < 0.0001; unpaired t test with Welch’s correction compared to the 0 μmol L−1 controls