Fig. 3

Hs578T/NOD1 and Hs578T/NOD2 cell populations display higher viability in solid substrate and in soft agarose, and this effect is enhanced by NOD1/2 agonists. Colony formation assays, comparing the total number of colonies per well, from wild-type or transduced Hs578T populations overexpressing GFP, NOD1 or NOD2. a Images of colonies formed by the different Hs578T populations in solid substrate upon staining with crystal violet. b Total number of colonies formed per well in both b solid and c soft agarose substrates, by transduced Hs578T populations in comparison to the wild-type. In both assays, Hs578T/NOD1 and Hs578T/NOD2 populations were treated with 5 μg/mL γ-tri-DAP and MDP (Invivogen), respectively (unpaired student’s t-test; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. a n = 3; b n = 4)