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Fig. 1 | BMC Research Notes

Fig. 1

From: Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase

Fig. 1

a Nucleotide sequence and translation of the N-terminal Avi-TEV-3xFLAG tandem affinity tag, cloned as an Acc65I/XhoI fragment in pBluescript SK (see also Additional file 1: Figure S1A). The Kozak sequence is underlined. b Nucleotide sequence and translation of the C-terminal 3xFLAG-TEV-Avi tandem affinity tag which was cloned as an EcoRI/HindIII fragment in pBluescript KS (see also Additional file 1: Figure S1B). Asterisks denote stop codons

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BMC Research Notes

ISSN: 1756-0500

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