Fig. 2
Aged Cav1-transgenic mice exhibit an altered splenic microenvironment. a Representative H&E stained sections of Cav1+/+, Cav1+/−, and Cav1−/− spleens at ×4 magnification. b Bar graph of the mean splenic secondary follicle area (μm2) for listed genotypes at 12 and 36 weeks (n = 5–10 follicles across n = 2–4 mice per group). c Percentage of observable secondary follicles for listed genotypes at 12 and 36 weeks (n = 2–4 per group). d Representative Ki67 and Movat’s Pentachrome stained histological sections from 36-week spleens for listed genotypes at 20× magnification (n = 3 per group). Increased fibrin staining is visualized in bright red. e Bar graph of mean plasma cells identified per field of view (×40) for listed genotypes from 36-week splenic sections (n = 3 per group). (Bar graphs show mean ± SD, with each dot corresponding to a biological replicate NS not significant, *p < 0.05 ANOVA and Tukey post hoc test)