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Fig. 1 | BMC Research Notes

Fig. 1

From: A conditional Pax6 depletion study with no morphological effect on the adult mouse corneal epithelium

Fig. 1

Pax6 and keratin 12 immunohistochemistry of corneal epithelium after a 6-week chase period. al Central region of the adult corneal epithelium, immunostained for Pax6 (brown DAB endpoint) and counterstained with haematoxylin, showing various controls with different genotype and treatment combinations, 6 weeks after treatment (ag), and several examples of weak and/or patchy staining of CAG-CreERTg/−;Pax6fl/+corneas, 6 weeks after treating with tamoxifen (hl). mt Central region of the adult corneal epithelium, immunostained for keratin 12 (brown DAB endpoint) and counterstained with haematoxylin, showing various controls with different genotype and treatment combinations, 6 weeks after treatment (ms), and a CAG-CreERTg/−;Pax6fl/+ cornea, 6 weeks after treating with tamoxifen (t). Scale bars = 50 μm. ce corneal epithelium, CO corn oil treatment, cs corneal stroma, NT no treatment, Tx tamoxifen treatment. All mice were on a predominantly CBA/Ca genetic background

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