Fig. 3

a Optimized SCAR-PCR assay using recombinant pDrive plasmid as DNA template. Optimum annealing temperature was 56 °C. M: 100 bp and 1 Kbp ladders, respectively. b Gel showing results of PCR assay using specific SCAR primer. M: 100 bp and 1 Kbp ladders, respectively