Fig. 3
From: Immune adaptor protein SKAP1 (SKAP-55) forms homodimers as mediated by the N-terminal region
Confirmation of dimerisation with anti-SKAP1 blotting. L293T cells transfected with GFP-SKAP (lane 1), Flag-SKAP1 (lane 2), FlagDM*SKAP1 + Flag SKAP1 (lanes 3), Flag-SKAP1 and SKAP-GFP + (lanes 4) and GFP-SKAP1 + FlagDM*SKAP1 (lanes 5) (left panel). Right panel: anti-Flag precipitates from lysates in left panel. Lower insert shows a longer exposure of a section of lanes 4 and 5