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Fig. 1 | BMC Research Notes

Fig. 1

From: Myeloid differentiation primary response protein 88 (MyD88)-deficient dendritic cells exhibit a skewed cytokine response to BCG

Fig. 1

Cytokine secretion by MyD88−/− dendritic cells (DCs) in response to BCG. Bone marrow-derived DCs from wild-type (open bars) and MyD88−/− mice (filled bars) were stimulated with BCG at MOI of 10. Culture supernatants were harvested after 24 h and analyzed for TNF-α, IL-12p40. Significant levels of TNF-α and IL-12p40 were produced by BCG-stimulated wild-type and MyD88−/− DCs, compared with unstimulated DCs (a, b). Culture supernatants were also analyzed for IL-10 levels. Significant amount of IL-10 was secreted by BCG-stimulated wild-type, but not by MyD88−/− DCs (c). Synthetic TLR2 ligand Pam3CSK4 (100 ng/ml) was used as an experimental control. Data shown are mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001. ns not significant

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