Fig. 1

Cytokine secretion by MyD88^−/− dendritic cells (DCs) in response to BCG. Bone marrow-derived DCs from wild-type (open bars) and MyD88^−/− mice (filled bars) were stimulated with BCG at MOI of 10. Culture supernatants were harvested after 24 h and analyzed for TNF-α, IL-12p40. Significant levels of TNF-α and IL-12p40 were produced by BCG-stimulated wild-type and MyD88^−/− DCs, compared with unstimulated DCs (a, b). Culture supernatants were also analyzed for IL-10 levels. Significant amount of IL-10 was secreted by BCG-stimulated wild-type, but not by MyD88^−/− DCs (c). Synthetic TLR2 ligand Pam₃CSK₄ (100 ng/ml) was used as an experimental control. Data shown are mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001. ns not significant