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Table 1 Catalytic parameters and specificity constant of Dr-dUTPase for different nucleotides

From: Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis

Nucleotides Km (μM) kcat (s−1) kcat/Km (M−1 s−1)
dUTP (n = 7) 4.0 ± 0.4 3.4 × 10 ± 3.0 8.5 × 106 ± 1.1 × 106
dATP 9.4 × 10 ± 1.2 1.5 ± 0.2 1.6 × 104 ± 2.1 × 103
dTTP 1.1 × 102 ± 7.2 1.4 ± 0.3 1.3 × 104 ± 2.8 × 103
dCTP 7.7 × 10 ± 3.5 1.6 ± 0.2 2.1 × 104 ± 2.7 × 103
dGTP 2.1 × 102 ± 1.0 × 10 2.9 ± 0.5 1.4 × 104 ± 2.4 × 103
dUTP, 5 mM MnCl2 7.3 ± 1.2 4.8 × 10 ± 6.8 6.5 × 106 ± 2.1 × 106
  1. Measurements were triplicated (n = 3) except in the case of dUTP with MgCl2