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Table 1 Catalytic parameters and specificity constant of Dr-dUTPase for different nucleotides

From: Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis

Nucleotides

Km (μM)

kcat (s−1)

kcat/Km (M−1 s−1)

dUTP (n = 7)

4.0 ± 0.4

3.4 × 10 ± 3.0

8.5 × 106 ± 1.1 × 106

dATP

9.4 × 10 ± 1.2

1.5 ± 0.2

1.6 × 104 ± 2.1 × 103

dTTP

1.1 × 102 ± 7.2

1.4 ± 0.3

1.3 × 104 ± 2.8 × 103

dCTP

7.7 × 10 ± 3.5

1.6 ± 0.2

2.1 × 104 ± 2.7 × 103

dGTP

2.1 × 102 ± 1.0 × 10

2.9 ± 0.5

1.4 × 104 ± 2.4 × 103

dUTP, 5 mM MnCl2

7.3 ± 1.2

4.8 × 10 ± 6.8

6.5 × 106 ± 2.1 × 106

  1. Measurements were triplicated (n = 3) except in the case of dUTP with MgCl2