Fig. 2

Transfection efficiency of astrocytes in primary cortical cultures. a Representative images of transfected astrocytes (GFP⁺/GFAP⁺) in AE and in AC cultures. AE cultures transfected at 60–80% confluency in 6-well plates for the indicated periods and about 10 fields were imaged per well. AC cultures transfected at DIV2 for 48 h in 24-well plates and 7 fields were imaged per well. Each experiment was done in replicates (2 or 3 wells/condition). Total number of cells quantified in each experiment was; (3500–7800 cells) for AC and (2900–7800 cells) for AE. b Quantitative analysis of the percentage of GFP⁺/GFAP⁺ cells among the total DAPI⁺ cells (total population, blue). Mean transfection efficiency of astrocytes in AE was obtained from 3 and 2 independent cultures for 24 hpt and 48 hpt experiments, respectively. Mean transfection efficiency of astrocytes in AC was obtained from 2 independent cultures. Data represents mean value ± SEM