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Fig. 3 | BMC Research Notes

Fig. 3

From: Estimating transfection efficiency in differentiated and undifferentiated neural cells

Fig. 3

Transfection efficiency of neurons in primary cortical cultures. Representative images of transfected neurons (GFP+/MAP2+) in AC (a) and in AF (b) cultures. Cortical cultures were transfected at DIV7/8 in 24-well plates for the indicated periods. c Quantitative analysis of the percentage of GFP+/MAP2+ cells among the total DAPI+ cells (total population, blue). The transfection rate for AC was 1.3% ± 0.3 at 24 hpt and 2.6% ± 0.4 at 48 hpt. About 6–9 fields were imaged per well and each experiment was done in triplicates (3 wells/condition). Mean transfection efficiency of neurons in AC was obtained from 7 and 3 independent cultures for 24 hpt and 48 hpt experiments, respectively. About (5164–20,014 cells) were quantified in each experiment for AC. As for the AF transfection rate was 4.9% ± 1.5 at 24 hpt and 6.4% ± 1.9 at 48 hpt. Mean transfection efficiency of neurons in AF was obtained from 5 and 3 independent cultures for 24 hpt and 48 hpt experiments, respectively. About (476–6097 cells) were quantified in each experiment for AF. Data represents mean value ± S.E.M

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