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Table 1 Comparison of RT-PCR and culture-based methods of S. aureus and MRSA in DFUs

From: Bacterial isolation and antibiotic susceptibility from diabetic foot ulcers in Kenya using microbiological tests and comparison with RT-PCR in detection of S. aureus and MRSA

Species

Reference

Assay

Sensitivity (%)

Specificity (%)

PPV (%)

NPV (%)

S. aureus

Culture

RT-PCR

90.9

82.5

58.8

97

RT-PCR

Culture

58.8

97

90.9

66.7

MRSA

Culture

RT-PCR

 

72.7

 

100

RT-PCR

Culture

 

100

 

72.7

  1. In this Table, both culture-methods and RT-PCR were used as gold standards. In the column heading, the gold standard is the “Reference” while the method being tested, the “Assay”