Fig. 1

a Heatmap of the most regulated genes (all genes with absolute log2fold-change ≥ 1 and adjust p-value ≤ 0.01) in the corpus callosum (CC), cerebellum (Cb) and cortex (Cx) at different ages [postnatal day (P)7, 10, 42]. b Representative immunofluorescent images of Cx3cr1^GFP/WT mice at P7 showing microglia (green) with a ramified morphology in the cortex (yellow error) and amoeboid microglia (white errors) in the CC and Cb. Scale bar 200 µm; blue = DAPI. c Quantification of CX₃CR1⁺ microglia in the CC, Cx and Cb at P7, P10 and P21; N = 3 mice per timepoint