Fig. 2

Gsr gene is modulated in ESCs along a neural progenitor differentiation protocol. 46C ESCs were subjected to a neural progenitor differentiation protocol. a Representative picture of cells at day 6 of differentiation showing expression of GFP, reporter of Sox1 promoter activity. Phase contrast, left panel; GFP, right panel. Scale bars: 100 µm. b Representative immunostaining of Nanog for 46C ESCs at days 0 (D0) and 3 (D3) of the neural progenitor differentiation protocol. Nuclei were counterstained with DAPI. Scale bars: 20 μm. The nuclear intensities were quantified and represented as dot plots, mean ± SEM are indicated, *p < 0.05. c RNA was extracted at days 0 (D0), 1 (D1), 3 (D3) and 6 (D6) after the induction of differentiation and mRNA levels of the indicated genes were measured by RT-qPCR. Gene expression was normalized to the geometrical mean of Gapdh and Pgk1 expression and referred to the control condition (D0). Results are shown as mean ± SEM of three independent experiments. Different letters indicate statistically significant differences between treatments (p < 0.05)