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Table 3 Biospecimen type and testing plan

From: Respiratory health, allergies, and the farm environment: design, methods and enrollment in the observational Wisconsin Infant Study Cohort (WISC): a research proposal

Sample type

Testing plan

Maternal vaginal swab

Vaginal microbiome

Maternal breast milk

sIgA, milk microbiome, metabolomics

Infant/child blood

Innate immune cell function, Treg cell profile, plasma lipidomics, allergen-specific and total IgE, cryopreservation

Infant/child nasal swab

Nasal microbiome, respiratory virus detection (surveillance)

Infant/child nasal illness swab

Respiratory virus detection (illness)

Child nasal brushinga

Transcriptomics

Infant/child stool

Gastrointestinal microbiome

Infant/child urine

Metabolomics

Infant/child skin swab

Skin microbiome

Infant/child saliva

Oral microbiome

Household airborne and vacuum dust

Environmental microbiome (bacterial and fungal)

Drinking water

Environmental microbiome

Barn airborne dust

Environmental microbiome (bacterial and fungal)

Farm milk

Microbiome, metabolomics

  1. sIgA secreted immunoglobulin A, Treg T regulatory cell
  2. aObtained in subset of participants