Fig. 1From: Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assayOptimization of the LAMP assay. a The LAMP assay was performed using the LAMP primer set with or without a loop primer. b The LAMP reaction was performed at 61, 63, 65, and 67 °C. Amplification profiles were obtained from positive control (PC) samples using Aconitum. japonicum subsp. subcuneatum DNA (5 ng) and negative control (NC) samples using Anemone flaccida DNA (5 ng)Back to article page