Table 1 Results of LAMP detection assay
From: Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay
Sample | DNA template | Reaction timea | |
|---|---|---|---|
Sensitivity | Ac. japonicum subsp. subcuneatum | 10 ng | 6.8 ± 0.2 min |
1 ng | 8.0 ± 0.3 min | ||
100 pg | 8.9 ± 0.2 min | ||
10 pg | 10.3 ± 0.4 min | ||
1 pg | 11.9 ± 0.3 min | ||
100 fg | nd | ||
(No template) | nd | ||
Specificity | Ac. japonicum subsp. subcuneatum | 5 ng | 7.1 ± 0.1 min |
Ac. sachalinense subsp. yezoense | 5 ng | 7.3 ± 0.0 min | |
Ac. chinense | 5 ng | 7.2 ± 0.2 min | |
Anemone flaccida | 5 ng | nd | |
Parasenecio delphiniifolius | 5 ng | nd | |
Mixture | Ac. japonicum subsp. subcuneatum | 50 pg | 10.0 ± 0.2 min |
Ac. japonicum subsp. subcuneatum + Anemone flaccida | 50 pg + 50 ng | 9.8 ± 0.3 min | |
Anemone flaccida | 50 ng | nd |