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Table 1 Results of LAMP detection assay

From: Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

  Sample DNA template Reaction timea
Sensitivity Ac. japonicum subsp. subcuneatum 10 ng 6.8 ± 0.2 min 
1 ng 8.0 ± 0.3 min
100 pg 8.9 ± 0.2 min 
10 pg 10.3 ± 0.4 min 
1 pg 11.9 ± 0.3 min 
100 fg nd
  (No template)   nd
Specificity Ac. japonicum subsp. subcuneatum 5 ng 7.1 ± 0.1 min 
Ac. sachalinense subsp. yezoense 5 ng 7.3 ± 0.0 min 
Ac. chinense 5 ng 7.2 ± 0.2 min 
Anemone flaccida 5 ng nd
Parasenecio delphiniifolius 5 ng nd
Mixture Ac. japonicum subsp. subcuneatum 50 pg 10.0 ± 0.2 min 
Ac. japonicum subsp. subcuneatum + Anemone flaccida 50 pg + 50 ng 9.8 ± 0.3 min  
Anemone flaccida 50 ng nd
  1. nd not detected
  2. aThe reactions were carried out in triplacates