Fig. 1
From: Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Volumetric activity (green diamond) and biomass yields (dry cell weight per litre) (black circle) during bioreactor cultivations of each target protein. Arrows indicate the initiation of the glycerol fed-batch phase (20–22 h), where a 50% (w.v−1) glycerol feed supplemented with trace salts was added at a rate of 72.6 mL/h. a Glucuronoyl esterase (GE) b Cellobiose Dehydrogenase (CDH). Error bars indicate standard deviation from mean of three fermentation replicates. c SDS-PAGE analysis of final fermentation samples (each lane representing one replicate of a bioreactor fermentation) of 20 μL supernatant containing the target proteins. Lane 1: Protein molecular weight marker. Lane 2–4: CDH-containing supernatant (rCDH, 127 KDa). Lanes 5–7: Glucuronoyl esterase-containing supernatant (rGE, 78 KDa)