Table 1 Summary of enzymatic yields, kinetics and productivity for bioreactor cultivations of recombinant P. pastoris at the end of the glycerol fed-batch stage, and comparisons to previous expression attempts
From: Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Parameter | GE (this study) | GE (previous studies) | Reference | CDH (this study) | CDH (previous studies) | Reference |
|---|---|---|---|---|---|---|
Highest Volumetric Activity (U L−1) | 238.17 | 5.7a | Topakas et al. [5] | 329.49 | 7955a | Stapleton et al. [26] |
Highest Recombinant Protein Titre (mg L−1) | 2778.01 | NR | – | 1489.30 | 633b | Ma et al. [28] |
% recombinant protein vs total protein in supernatant | 93.61% | – | 50.89% | NR | – | |
Biomass concentration (gDCW L−1) | 120.94 | – | 136.47 | – | ||
μmax (h−1) | 0.15 | – | 0.16 | – | ||
qp.max (mg recombinant protein.[g biomass∙h]−1) | 1.22 | – | 0.76 | – | ||
qp.mean (mg recombinant protein.[g biomass∙h]−1) | 0.52 | – | 0.43 | – |