Table 2 Summary of volumetric activity and protein concentration values at successive steps of tangential flow filtration concentration of cell-free extracts obtained from bioreactor fermentations
From: Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Step | Volumetric activity (U L−1) | Total activity (U) | Yield (%, volumetric activity) | Total protein concentration (g L−1) | |
|---|---|---|---|---|---|
GE | CFE | 203.92 | 407.84 | 100.00 | 2.93 |
MFP | 196.07 | 352.93 | 86.54 | – | |
UFR | 1019.61 | 203.92 | 50.00 | 8.31 | |
UFP | 2.11 | 2.95 | – | – | |
CDH | CFE | 40.77 | 81.54 | 100.00 | 2.97 |
MFP | 39.34 | 70.81 | 86.84 | – | |
UFR | 202.08 | 40.42 | 49.57 | 10.16 | |
UFP | 0.03 | 0.04 | – | – |