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Table 2 Summary of volumetric activity and protein concentration values at successive steps of tangential flow filtration concentration of cell-free extracts obtained from bioreactor fermentations

From: Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris

  Step Volumetric activity (U L−1) Total activity (U) Yield (%, volumetric activity) Total protein concentration (g L−1)
GE CFE 203.92 407.84 100.00 2.93
MFP 196.07 352.93 86.54
UFR 1019.61 203.92 50.00 8.31
UFP 2.11 2.95
CDH CFE 40.77 81.54 100.00 2.97
MFP 39.34 70.81 86.84
UFR 202.08 40.42 49.57 10.16
UFP 0.03 0.04
  1. CFE cell-free extract, MFP microfiltration permeate, UFR ultrafiltration retentate or concentrated enzyme, UFP ultrafiltration permeate