Fig. 2
From: Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures
Monitoring Mycoplasma elimination by direct qPCR. Mycoplasma contaminated U937 cells were treated with Bio-Rad Mycoplasma Removal Agent at 0.5 μg/ml concentration. a A comparison of qPCR Ct values in the absence and presence of Mycoplasma Removal Agent in the medium of contaminated U937 cells. Student’s t-test was applied to compare the Ct values of removal agent containing samples with those of removal agent free samples (n = 3). b The first 4 days of treatment monitored by direct qPCR is shown (n = 4 at each time point). The Mycoplasma genome concentration on day 0 was defined as 100%