Fig. 2
From: Expression of a recombinant bacterial l-asparaginase in human cells
Glycosylation analysis of the bacterial l-Asparaginase expressed in the mammalian cell line HEK-293. a Predicted N-glycosylation sites in the l-Asparaginase sequence expressed in HEK-293 cells using the NetNGlyc 1.0 software. A position with the potential (vertical lines) crossing the threshold (horizontal line at 0.5) is predicted when glycosylated. b Immunodetection of recombinant l-Asparaginase expressed in HEK-293 cells and E. coli after deglycosylation with PNGase-F. (1) HEK-293 l-Asparaginase treated with PNGase-F, (2) HEK-293 l-Asparaginase untreated with PNGase-F, (3) E. coli l-Asparaginase treated with PNGase-F, (4) E. coli l-Asparaginase untreated with PNGase-F