Fig. 2

SB415286 decreases T-cell contacts with other cells. Inhibition of GSK-3 reduces the number of cell-to-cell contacts required to induce target killing. Quantification of contact times a total number of contacts for each condition, (Ova alone Mean = 72.67 ± 4.3, Plus SB415286 Mean = 40.00 ± 2.89). b GSK3 inhibition does not alter the dwell times of T-cells. % of contacts with different durations of contact is shown. Data shown is pooled from (N) = 3 independent experiments. n.s no significant difference. c Left panel, number of contacts by each individual target cell tracked (n = 20 Target cells (EL4-OVA)). Right panel, Mean number of contacts by individual target cells from (N) = 3 independent experiments (Ova alone Mean = 3.8 ± 0.15, Plus SB415286 Mean = 1.7 ± 0.10). * P < 0.05; ** P < 0.005; *** P < 0.0005