Table 3 Characteristics of eight microsatellite loci in two collections of Cicindela puritana
Locus | Primer sequences | Size range | Motif | Locus characteristic | CR n = 24 | LCP n = 20 |
|---|---|---|---|---|---|---|
CpuQ1 | F: GCGACTTATATACAGTTAGTGGTGT | 218–251 | AAT(13) | NA | 1.00 | 5.00 |
R: TGTCTAACAATTCTCTCGGATTGC | HO | 0.00 | 0.65 | |||
uHE | 0.00 | 0.71 | ||||
AE | 1.00 | 3.23 | ||||
Microchecker null | No | No | ||||
Micorochecker scoring error | No | No | ||||
HWE P-value | NA | 0.6889 | ||||
CpuQ2 | F: ATAACGGGACACTGTGGACT | 135–183 | AAT(12) | NA | 4.00 | 6.00 |
R: ACACTTTGGCATTCAATTCGGA | HO | 0.50 | 0.30 | |||
uHE | 0.66 | 0.74 | ||||
AE | 2.81 | 3.57 | ||||
Microchecker null | Yes | Yes | ||||
Micorochecker scoring error | No | No | ||||
HWE P-value | 0.1688 | 0.0000 | ||||
CpuQ3 | F: CTTCGTACGTCATGAAAGTACTTAT | 196–214 | ACT(12) | NA | 3.00 | 4.00 |
R: AACTTCAAGCTTTCTGGATCAGA | HO | 0.60 | 0.40 | |||
uHE | 0.50 | 0.38 | ||||
AE | 1.97 | 1.58 | ||||
Microchecker null | No | No | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | 1.0000 | 0.4148 | ||||
CpuQ10 | F: AAATTACGCGCGTGTACTGC | 124–136 | ATC(11) | NA | 2.00 | 4.00 |
R: AAGGGCTGATTCACGACACC | HO | 0.05 | 0.50 | |||
uHE | 0.05 | 0.56 | ||||
AE | 1.05 | 2.19 | ||||
Microchecker null | No | No | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | NA | 0.7256 | ||||
CpuQ13 | F: AGTTTCGCCACAAATCCTGC | 116–140 | AAT(10) | NA | 5.00 | 3.00 |
R: GGTAGGACCACCGCAGAATC | HO | 0.75 | 0.25 | |||
uHE | 0.68 | 0.66 | ||||
AE | 2.99 | 2.83 | ||||
Microchecker null | Yes | Yes | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | 1.0000 | 0.0006 | ||||
CpuQ19 | F: AGCAGCCACCTCTCTACACA | 156–168 | ACAT(9) | NA | 3.00 | 3.00 |
R: AGAGATATGTAGCCGGAAAGTAGC | HO | 0.20 | 0.15 | |||
uHE | 0.41 | 0.44 | ||||
AE | 1.65 | 1.75 | ||||
Microchecker null | Yes | Yes | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | 0.0053 | 0.0008 | ||||
CpuQ23 | F: TGATATGTGTTGACTTGGTGTAATG | 146–162 | ACTAT(8) | NA | 3.00 | 2.00 |
R: ACCATAATGCAACTTTATACATATGCT | HO | 0.60 | 0.45 | |||
uHE | 0.65 | 0.50 | ||||
AE | 2.75 | 1.96 | ||||
Microchecker null | No | No | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | 0.2368 | 0.6748 | ||||
Cpu31 | F: ATGATCTCCCGGTCTGTCCT | 152–192 | AAAT(7) | NA | 3.00 | 2.00 |
R: AATGTTCATTGATGTACTCGATCT | HO | 0.35 | 0.05 | |||
uHE | 0.30 | 0.05 | ||||
AE | 1.42 | 1.05 | ||||
Microchecker null | No | No | ||||
Microchecker scoring error | No | No | ||||
HWE P-value | 1.0000 | 0.0000 | ||||
NA (mean, SE) | 3.00, 0.42 | 3.63, 0.50 | ||||
HO (mean, SE) | 0.38, 0.10 | 0.34, 0.07 | ||||
uHE (mean, SE) | 0.41, 0.10 | 0.50, 0.08 | ||||
AE (mean, SE) | 1.95, 0.28 | 2.27, 0.31 | ||||
HWE P-value (Fisher’s method) | 0.1523 | 0.0000 |