Fig. 1

Biofilm formation in the presence of divalent chelators Biofilms were incubated in RPMI-plus-MOPS medium with the following cation chelators: aN,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine, b deferoxamine, c triethylenetetramine, and d ethylenediaminetetraacetic acid. Controls included the same amount of each solvent without chelators. Biofilm formation was measured using the XTT reduction assay. Measurements were performed four times under each condition, and data are expressed as means ± standard deviations. *P < 0.05 compared to controls