A recombinant antibody toolbox for Dictyostelium discoideum

BMC Research Notes

Table 1 List of sequenced hybridomas; highlighted in bolditalic, the antibodies converted to rAbs

ABCD_^a	Hybridoma	UniProt	DDB_	Target	Original ref.^b	rAb ref.^b
AN700	H9	–	–	Membrane protein p23	[11]
*AJ155*	H194	–	–	Membrane protein p23	[11]	[12]
*AJ513*	H72	–	–	Membrane protein p25	[11]	[13]
AN701	H36	–	–	Surface protein p46	[14]
*AJ514*	221-342-5	–	–	Common antigen 1 (CA1)	[15]	[16, 17]
AN704	173-185-1	–	–	Common antigen 1 (CA1)	[18]
*AK426*	1/39	–	–	Golgi	[19]	[20]
*AK423*	224-236-1	P07830	several	Actin	[21]	[22, 23]
AK566	33-294-17	P08796	G0289073	Contact site A protein	[24]
AN709	41-71-21	P08796	G0289073	Contact site A protein	[24]
AK425	176-3-6	P27133	G0267382	Coronin A	[25]
AN706	194-62-7	P27133	G0267382	Coronin A	[26]
AN707	130-80-2	P21837	G0285419	Crystal protein	[27]
AN710	80-52-13	P02886	G0273063	Discoidin 1 chain A	[28]
*AJ154*	H161	Q7YXD4	G0287297	Endosomal membrane protein p80	[11]	[29, 30]
AN702	H191	Q7YXD4	G0287297	Endosomal membrane protein p80	[11]
*AK421*	70-100-1	Q01501	G0271848	Porin A	[31]	[32, 33]
AK424	21-55-4	P08799	G0286355	Myosin II heavy chain	[34]
AN708	21-96-3	P08799	G0286355	Myosin II heavy chain	[35]
AK567	221-64-1	Q86IA3	G0276141	Protein disulfide isomerase	[36]	c
AN703	221-42-1	Q86IA3	G0276141	Protein disulfide isomerase	[36]
*AK422*	B4.2	O77257	G0278725	Secreted protein SctA	[37]	[38]
*AJ156*	169-477-5	P0CE95	G0290481	Talin A	[39]	[40, 41]
AN705	227-341-4	P0CE95	G0290481	Talin A	[42]
*AJ520*	221-35-2	P54647	G0287127	V-ATPase subunit A	[43]	[44, 45]
AJ515	224-256-2	P54648	G0284473	V-ATPase subunit C	[46]	c

^a ABCD nomenclature (https://web.expasy.org/abcd/); the ABCD database is a manually curated repository of sequenced antibodies [47]
^b “Original references” correspond to the first descriptions of a monoclonal antibody. “rAb references” describe the characterization of the converted rAbs
^c The recombinant versions of these antibodies were not produced efficiently in our hands

ISSN: 1756-0500