Table 2 Sequences of Tpi primers used in this study
From: Multiplex PCR for identification of two butterfly sister species: Eurema mandarina and Eurema hecabe
Primer | Sequence | Target species | Reference |
|---|---|---|---|
Em4-F | 5′–GGCTCCAACAATTGGGAGATTA–3′ | Eurema mandarina | This study |
Em4-R | 5′–TACAGGCAATGACCTTGAGGC–3′ | ||
Eh6-F | 5′–TGTGGCCTTCTGCCCTATTAAA–3′ | Eurema hecabe | This study |
Eh6-R | 5′–ACAGGCAATGACCTTGAGTC–3′ | ||
Tpi-F | 5′–GGTCACTCTGAAAGGAGAACCACTTT–3′ | Universal (Lepidoptera) | Jiggins et al. [26] |
Tpi-R | 5′–CACAACATTTGCCCAGTTGTTGCAA–3′ |