Skip to main content

Table 2 The brine shrimp cytotoxicity profile of Naja ashei venom, two commercial antivenoms, and vincristine sulphate (standard cytotoxic agent)

From: Enzymatic activity and brine shrimp lethality of venom from the large brown spitting cobra (Naja ashei) and its neutralization by antivenom

Sample Duration of exposure Mortality per test dose LC50 (µg/ml) Toxicity
10 µg/ml 100 µg/ml 1000 µg/ml Meyer’s toxicity index [16] Clarkson’s toxicity index [20]
Vincristine sulphate (positive control) 24 0 30 46 171.83 Toxic Highly toxic
48 35 50 50 2.10 Toxic Highly toxic
72 50 50 50 All died Toxic Highly toxic
Antivenom I 24 0 0 0 No mortality Non toxic Non toxic
48 11 8 29 2346.23 Non toxic Non toxic
72 13 9 29 5268.05 Non toxic Non toxic
Antivenom II 24 0 0 0 No mortality Non toxic Non toxic
48 11 8 13 599,484,250.30 Non toxic Non toxic
72 12 11 17 1622.89 Non toxic Non toxic
Naja ashei venom 24 0 48 50 63.02 Toxic Highly toxic
48 26 50 50 4.73 Toxic Highly toxic
72 40 50 50 0.15 Toxic Highly toxic