Fig. 2

The regulatory genes klf9 and fkbp5 have higher on average activity in blood cells of adults derived from cortisol-treated embryos. a ATAC-seq peaks associated with klf9 and fkbp5, from three biological replicates of each treatment (r1-r3). For each replicate sample blood was pooled from 6 individuals mixed sex with equal representation of males and females. b Relative expression of klf9 and fkbp5 in 17 blood samples of adults from different experimental cohorts of cortisol-treated embryos compared to their control siblings sampled at the same time. The averages ± SEM are also shown. For each experimental sample blood was pooled from 6 individuals of each group (control and treated), of mixed sex with equal representation of males and females. c ChIP-qPCR of H3K4me3 levels in the promoter regions of klf9 and fkbp5 from a single sample of blood pooled from 6 individuals (mixed sex, equal representation). The averages ± SD of three replicate qPCR measurements (technical replicates) are shown. d Relative expression of klf9 and fkbp5 in blood cells immediately after being drawn, and then after 6 or 24 h of ex vivo culture in the absence of cortisol. The plots represent the grand means ± the SEM of three biological replicates, each done on blood samples pooled from 6 individuals of mixed sex