Fig. 2

DUSP6 protein is over-expressed in human glioblastomas. a Western Blot analysis of DUSP6, p-ERK and ERK in primary GBM samples. In both panels CTRL+ indicates positive immune-reactive control obtained with U87MG infected with the adenovirus DUSP6. b Western Blot analysis of DUSP6, p-ERK and ERK in U87MG, U251MG, T98G and MCF7 cell lines. The corresponding bar graphs show relative expression of proteins normalized to α-tubulin. c, d Post-translational regulation of DUSP6 protein levels- U87MG and U251MG cell lines were treated with cycloheximide (CHX, 20 µg/mL) for the time indicated and the decay of the target proteins over time was determined by Western Blot with specific antibodies. The corresponding bar graphs show relative expression of proteins normalized to α-tubulin