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Table 1 Primer sequences for PCR and LAMP diagnostic marker sets of MS1 in Cryptomeria japonica

From: Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

Maker ID

Primer ID

Primer sequencea

(a) ALP_ms1-1

CJt020762_F145

CCATGCCTTTCTTACATGACGAG

CJt020762_R294_A

GCCTCCCTCGCGCCATTGATTAATGGGAAAGCCCAGAA

(b) ALP_ms1-2

CJt020762_F1O7.F_A

GCCTCCCTCGCGCCACCTCCGGTGTATCAAACTTCAA

CJt020762_F1O7.R

ATTCGCCCTTTCCAAATGTTAGC

(c) ALP_ms1

CJt020762_ms1-1_F

GCTATAGACTGCACCGACCC

CJt020762_ms1-1_R

AGCCCAGAAAATCGTCCCTG

CJt020762_ms1-2_F

GAATCCACCGCCACGACTAT

CJt020762_ms1-2_R

TGAACTCTGTTTCCATGGCA

(d) ASP_ms1-1

CJt020762_mt-224F_C.f

CTGTGACTCACTGGCCACAGTAAC

Cjt020762common.r

AGAGAGTGATGGTTTGATTAATGGGAAA

(e) ASP_ms1-1_wt

CJt020762_wt-227F_A.f

GTGACTCACTGGCCACAGTCATAAA

Cjt020762common.r

AGAGAGTGATGGTTTGATTAATGGGAAA

(f) LAMP_ms1-1

4D-1_FIP

ACGATGGGGTCGGTGCAGTCTAGCGGTAGCAGAAAGTGTGAT

4D-1_BIP-3C

ACGAGATCAGCCAAGCTTTCCAACCCTGCGTGGGTCTG

4D-1_F3

GCGGCAATTGTGAGAGCATT

4D-1_B3

GAAAATCGTCCCTGGAGACG

(g) LAMP_ms1-1_wt

MS1-190727_FIP-2A

TATTGATGACTGTGGCCAGTGAAGATCAGCCAAGCTTTCCAT

MS1-190727_BIP

GGTGCTTGTGCGAGCTCGTCGGAGCGAGAGAGTGATGGTT

MS1-190727_F3

ATCGTGAGCCTCTCACCA

MS1-190727_B3

GGTCAAGTTCACGCGGATA

(h) LAMP_ms1-2

30D-1-FIP-3T

TGTAGTGATCCCCAAAATAGCCGTTAGCATTGCTGGAGCC

30D-1-BIP

TGAAGTTTGATACACCGGAGGTTTCTGCTAAATATAACTTTGAACTCTG

30D-1-F3

TGATTCGCCCTTTCCAAAT

30D-1-B3

AGACAACAGCAATTCATTCA

  1. aBold and underlined fonts indicate artificial mismatch introduced to increase specificity