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Table 1 Effect of different antibiotic treatments on attached B. burgdorferi biofilm mass at 10 µM and 50 µM concentration evaluated with crystal violet biofilm assay after 72 h

From: Effect of dapsone alone and in combination with intracellular antibiotics against the biofilm form of B. burgdorferi

Antibiotics single 10 µM residual %  ± %SD 50 µM residual %  ± %SD Antibiotics dual combinations 10 µM residual %  ± %SD 50 µM residual  ± %SD
Control (PBS) 100% 100% Control (PBS) 100% 100%
DDS 69% ± 5.1 58% ± 4.7 DDS + DOXY 68% ± 5.3 65% ± 4.6
DOXY 107% ± 8.2 103% ± 11.6 DDS + RIF 82% ± 7.6 71% ± 5.6
RIF 76% ± 4.3 60% ± 8.9 DDS + AZ 106% ± 9.1 93% ± 7.3
CEF 109% ± 8.6 102% ± 9.5 DDS + CEF 83% ± 7.3 101% ± 10.3
AZ 101% ± 7.4 91% ± 7.9 DOXY + RIF 73% ± 5.8 74% ± 6.5
Antibiotics triple combinations 10 µM residual %  ± %SD 50 µM residual %  ± %SD Antibiotics quadruple combinations 10 µM residual %  ± %SD 50 µM residual %  ± %SD
Control (PBS) 100% 100% Control (PBS) 100% 100%
DDS + DOXY + RIF 78% ± 5.8 52% ± 4.1 DDS + DOXY + RIF + CEF 72% ± 5.2 79% ± 6.2
DDS + DOXY + CEF 65% ± 4.2 60% ± 8.8 DDS + DOXY + RIF + AZ 78% ± 5.4 58% ± 3.5
DDS + DOXY + AZ 80% ± 7.7 72% ± 6.5 DDS + RIF + AZ + CEF 81% ± 6.8 68% ± 5.5
DDS + RIF + AZ 69% ± 4.2 77% ± 8.5 DOX + RIF + AZ + CEF 98% ± 8.5 82% ± 5.1
DDS + RIF + CEF 70% ± 5.6 62% ± 5.6    
  1. Table summarizes the mean % of residual viability with ± SD compared to the PBS treated control. N = 20
  2. DDS dapsone, DOXY doxycycline, RIF rifampin, CEF cefuroxime, AZ azithromycin