Table 1 Effect of different antibiotic treatments on attached B. burgdorferi biofilm mass at 10 µM and 50 µM concentration evaluated with crystal violet biofilm assay after 72 h
Antibiotics single | 10 µM residual %  ± %SD | 50 µM residual %  ± %SD | Antibiotics dual combinations | 10 µM residual %  ± %SD | 50 µM residual  ± %SD |
|---|---|---|---|---|---|
Control (PBS) | 100% | 100% | Control (PBS) | 100% | 100% |
DDS | 69% ± 5.1 | 58% ± 4.7 | DDS + DOXY | 68% ± 5.3 | 65% ± 4.6 |
DOXY | 107% ± 8.2 | 103% ± 11.6 | DDS + RIF | 82% ± 7.6 | 71% ± 5.6 |
RIF | 76% ± 4.3 | 60% ± 8.9 | DDS + AZ | 106% ± 9.1 | 93% ± 7.3 |
CEF | 109% ± 8.6 | 102% ± 9.5 | DDS + CEF | 83% ± 7.3 | 101% ± 10.3 |
AZ | 101% ± 7.4 | 91% ± 7.9 | DOXY + RIF | 73% ± 5.8 | 74% ± 6.5 |
Antibiotics triple combinations | 10 µM residual %  ± %SD | 50 µM residual %  ± %SD | Antibiotics quadruple combinations | 10 µM residual %  ± %SD | 50 µM residual %  ± %SD |
|---|---|---|---|---|---|
Control (PBS) | 100% | 100% | Control (PBS) | 100% | 100% |
DDS + DOXY + RIF | 78% ± 5.8 | 52% ± 4.1 | DDS + DOXY + RIF + CEF | 72% ± 5.2 | 79% ± 6.2 |
DDS + DOXY + CEF | 65% ± 4.2 | 60% ± 8.8 | DDS + DOXY + RIF + AZ | 78% ± 5.4 | 58% ± 3.5 |
DDS + DOXY + AZ | 80% ± 7.7 | 72% ± 6.5 | DDS + RIF + AZ + CEF | 81% ± 6.8 | 68% ± 5.5 |
DDS + RIF + AZ | 69% ± 4.2 | 77% ± 8.5 | DOX + RIF + AZ + CEF | 98% ± 8.5 | 82% ± 5.1 |
DDS + RIF + CEF | 70% ± 5.6 | 62% ± 5.6 |  |  |  |