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Table 2 Summary of the PCR profiles for cross-amplification of microsatellites in paca using 20 heterologous primer pairs developed for guinea pigs and capybara

From: Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance

PCR step Guinea pig Capybara
All CAPY1 CAPY9 and CAPY24 CAPY 4, CAPY6, CAPY7, and CAPY10 CAPY12
Initial denaturation 95 °C for 5 min 95 °C for 5 min
# of cycles 34 34 –-
Denaturation 95 °C for 40 s 94 °C for 30 s  
Annealing 55 °C for 30 s 53 °C for 30 s 54 °C for 30 s 55 °C for 30 s 57 °C for 30 s
Elongation 72 °C for 40 s 72 °C for 40 s
Final extension 72 °C for 5 min 72 °C for 30 min
Maintenance 4 °C 4 °C
  1. All guinea pig primers n = 12 (CUY5, CUY6, CUY7, CUY8, CUY9, CUY10, CUY17, CUY18, CUY22, CAVY2, CAVY11, and CAVY12; Aviles et al. [24] had the same PCR protocol. While capybara primers n = 8 (CAPY1, CAPY4, CAPY6, CAPY7, CAPY9, CAPY10, CAPY12 and CAPY24; Herrera et al. [26]) had similar conditions across the majority of the PCR profile, indicated by “–” in the table, they varied in annealing temperature