Fig. 3
From: Morphological study of embryonic Chd8+/− mouse brains using light-sheet microscopy
Quantification of apical cell morphology in wild-type [n = 1031] and Chd8+/− [n = 3854] E12.5 mouse brains. a, b 2.5D segmentation overlay of the apical surface of a Chd8+/− (HET) and b wild-type (WT) neurocortical epithelium. Scale bar: 20 µm. c Distribution of apical neighbour numbers per sample. The average number of cell neighbours is 5.84 for WT and 5.80 for HET, which is close to the topological requirement of 6. d Polygon type n times the mean polygon number of neighbours m of the cell n follows a linear relationship termed Aboave-Weaire’s Law. e Average apical cell area by cell neighbour number following a linear relationship termed Lewis’ Law (black line). f Cellular aspect ratios between their longest and shortest axis