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Table 1 Cytochrome c oxidase subunit I primer sequences used for the species-specific and universal barcode assays each forward and reverse primer was tailed with an M13 forward and reverse sequence respectively

From: The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs

Cocktail name

Ratio

Sequence (5′–3′)

References

C_BloodmealF2_t1

1

TCATTACAACWATTATYAAYATRAA

Lumsden et al. (in press)

1

TCATCACAACAGCAATYAAYATRAA

Mod.Mam.Rev_t1

1

TTCTCAACCAACCACAAAGACATTGG

Ivanova et al. [17]

1

TTCTCAACCAACCACAARGAYATYGG

3

TTCTCAACCAACCAIAAIGAIATIGG