Fig. 3

FACS quantification of blood samples taken from 16 week old mice with (Cre+) or without (Cre−) combined Hdac1 and 2 ablation. a Myeloid cells in peripheral blood: Cells were initially gated for CD45 and CD11b signal, percentages of CD45⁺ and CD11b⁺ cells on all cells are shown on the left. Of these CD45⁺ and CD11b⁺ cells, percentages of Ly6c-hi and Ly6c-low myeloid cells are shown. b Percentages of CD45⁺ and CD11b⁻ cells are shown on the left. Of these CD45⁺ and CD11b⁻ cells, percentages of CD19⁺ (B-) and CD3⁺ (T-)cells are shown next. To the right of these, the percentages of CD4 single-positive (SP), CD8 SP and CD4/CD8 double-positive (DP) cells compared to all CD3⁺ cells are depicted. No significant differences were found in either cell population, however some DP cells were seen in severely diseased mice. c FACS plot of a diseased Cre+ mouse, showing a large percentage of DP cells in blood, compared to a representative Cre− control