Fig. 2

Histologic damage scoring modified after Ziebart et al. [13, 14] evaluating 7 damage aspects (alveolar edema, interstitial edema, hemorrhage, inflammatory infiltration, epithelial destruction, microatelectasis and overdistension. Direct comparisons of samples from the cranial lobe (UL) and caudal lobe (LL) (A). Histone-treated animals showed no significant increase in lung damage independent of sample origin. A tendency towards higher scores in the dependent lung areas was not significant and mostly due to intraalveolar edema following instillation. Detailed damage aspect analysis (B) of pooled lung tissue samples. No significant shifts are seen for any damage criteria, suggesting the summative score is consistent. The intravenously administered animals were not analyzed since no pulmonary effects were seen