Fig. 3

MTT assay of breast cancer and MCF-10a cells following treatment with total lipoprotein lipid hydrolysis products generated by LPL. MCF-7, T47D, SKBR3, MDA-MB-231, MDA-MB-468 breast cancer cells and MCF-10a cells were treated with either total lipoprotein lipid hydrolysis products generated by LPL (HP) or mock heparinized media (Mock) for 24 h. At 4 h after the addition of MTT, the absorbance of the samples was read at 570 nm and 630 nm. Cellular metabolic activity was calculated by subtracting the 630 nm data from the 570 nm data. Data are presented as the mean of triplicate biological experiments with triplicate wells assessed per experiment, ± SD. *p < 0.05; **p < 0.01