Fig. 2
From: Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo
Analysis of Dll1AcGFPstopSF and Dll1SF mice. A Scheme of the modified 3’ region before (top) and after (bottom) Cre-mediated recombination. B Phenotype of wt (a), homozygous adult Dll1AcGFPHAstopSF (b) and Dll1SF (c) mice. C Skeletal preparations of wt (a), homozygous Dll1GFPHAstopSF (b) and Dll1SF d15.5 fetuses. D Detection of DLL1 variants in homozygous Dll1AcGFPHAstopSF, Dll1SF and wild type d10.5 embryos. DLL1 variants were immunoprecipitated with anti-HA (a), anti-GFP (b) or anti-Flag (c) antibodies and detected in Western blots using anti-DLL1antibody 1F9. + control: Lysate of DLL1 overexpressing ES cells. Arrows point to tagged DLL1 proteins. Photographs of the Western blot membranes are shown in Additional file 8: Figure S4. E GFP fluorescence in homozygous d9,5 Dll1AcGFPHAstopSF (a–a’’) and wild type (b–b’’) embryos; (a, b) bright field, (a’, b’) fluorescence, (a’’, b’’) overlay. Arrows in (a) point to irregular somites