Fig. 1
From: Immunomagnetic B cell isolation as a tool to study blood cell subsets and enrich B cell transcripts
Identification of a B cell gene signature. a Representative flow plots of PBMC immunophenotyping (top) and B cell purity check (bottom). Cells were previously gated on Lymphocytes/Single cells/Live/CD45+. b Summary of immunophenotyping results for PBMC samples. Individual values are displayed along with the average ± SD. c PCA plot from transcriptional analysis of whole blood (WB), PBMC and isolated B cells (BC). d List of 85 genes that make up the B cell gene signature. e Relative gene expression of select B cell signature genes. Statistics computed using unpaired T tests with correction for multiple comparisons. Graph displays average ± SD. p-value applies to comparisons of B cells versus both PBMC and WB. f. Heatmap with hierarchical clustering using the B cell gene signature. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001