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Table 1 RNA quality and NGS output among sample types

From: Immunomagnetic B cell isolation as a tool to study blood cell subsets and enrich B cell transcripts

RNA quality and yield Whole blood PBMC Isolated B cells
RIN 9.28 (0.3) 9.75 (0.3) 9.78 (0.2)
RNA yield (ng) 301 (30.9) 559 (364.2) 186 (137.4)
Sequencing output
 Total reads (× 106) 53.97 (7.9) 47.21 (2.45) 57.31 (4.86)
 Abundant reads (% of total) 7.60 (1.33) 7.46 (1.70) 9.87 (1.19)
 Aligned reads (% filtered) 97.60 (0.33) 97.88 (0.28) 94.93 (1.74)
 Fold coverage (coding) 113.32 (20.53) 103.92 (6.54) 100.69 (11.92)
  1. Values represent mean (SD), n = 4/group. RNA yield for whole blood corresponds to ng/200 µl, and for PBMC and B cells yield represents ng/1e6 cells. RIN, RNA integrity number