Table 1 RNA quality and NGS output among sample types
From: Immunomagnetic B cell isolation as a tool to study blood cell subsets and enrich B cell transcripts
RNA quality and yield | Whole blood | PBMC | Isolated B cells |
|---|---|---|---|
RIN | 9.28 (0.3) | 9.75 (0.3) | 9.78 (0.2) |
RNA yield (ng) | 301 (30.9) | 559 (364.2) | 186 (137.4) |
Sequencing output | |||
Total reads (× 106) | 53.97 (7.9) | 47.21 (2.45) | 57.31 (4.86) |
Abundant reads (% of total) | 7.60 (1.33) | 7.46 (1.70) | 9.87 (1.19) |
Aligned reads (% filtered) | 97.60 (0.33) | 97.88 (0.28) | 94.93 (1.74) |
Fold coverage (coding) | 113.32 (20.53) | 103.92 (6.54) | 100.69 (11.92) |