Rank | Methods |  | geNorm |  | NormFinder |  | Comparative ΔCq |  | BestKeeper | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ranking order | Ranking sum | Ranking order | Stability value (M) | Ranking order | Stability value | Ranking order | Stability value | Ranking order | Stability value (r) | SD (± Cq) | CV (% Cq) | |||||
Monocultured cementoblasts (OCCM-30) | ||||||||||||||||
 (1) | RPL22 | 13 |  | GUSB | 0.032 |  | TBP | 0.065 |  | RPL22 | 0.22 |  | PPIB | 0.750 | 0.470 | 2.278 |
 (2) | PPIB | 17 |  | POLR2A | 0.037 |  | PPIB | 0.065 |  | RPLP0 | 0.26 |  | YWHAZ | 0.685 | 1.541 | 7.321 |
 (3) | POLR2A | 21 |  | RPL22 | 0.042 |  | β-actin | 0.092 |  | POLR2A | 0.40 |  | GAPDH | 0.679 | 0.390 | 2.282 |
 (4) | RPLP0 | 23 |  | RPLP0 | 0.058 |  | RPL22 | 0.136 |  | GUSB | 0.40 |  | TBP | 0.620 | 0.503 | 2.121 |
 (5) | GUSB | 23 |  | UBC | 0.063 |  | RPL | 0.212 |  | UBC | 0.53 |  | RPL22 | 0.612 | 0.662 | 2.679 |
 (6) | β-actin | 24 |  | β-actin | 0.072 |  | RPLP0 | 0.538 |  | PPIB | 0.54 |  | β-actin | 0.453 | 0.543 | 3.208 |
 (7) | TBP | 24 |  | GAPDH | 0.083 |  | POLR2A | 0.719 |  | EEF1A1 | 0.59 |  | RPL | 0.172 | 0.204 | 0.790 |
 (8) | UBC | 27 |  | PPIB | 0.090 |  | GUSB | 0.831 |  | TBP | 0.59 |  | UBC | -0.038 | 0.489 | 1.756 |
 (9) | GAPDH | 32 |  | YWHAZ | 0.095 |  | UBC | 0.859 |  | β-actin | 0.64 |  | POLR2A | -0.115 | 0.197 | 0.875 |
 (10) | YWHAZ | 35 |  | EEF1A1 | 0.113 |  | EEF1A1 | 1.005 |  | RPL | 0.81 |  | GUSB | -0.282 | 0.331 | 1.393 |
 (11) | EEF1A1 | 39 |  | TBP | 0.183 |  | GAPDH | 2.421 |  | GAPDH | 2.12 |  | RPLP0 | -0.428 | 0.152 | 0.988 |
 (12) | RPL | - |  |  |  |  | YWHAZ | 2.461 |  | YWHAZ | 2.29 |  | EEF1A1 | -0.683 | 0.472 | 2.774 |
 (13) | TUBB | - |  |  |  |  |  |  |  | TUBB | 13.85 |  | TUBB | -0.986 | 0.295 | 0.795 |
Monocultured periodontal ligament cells (SV-PDL) | ||||||||||||||||
 (1) | GUSB | 14 |  | GUSB | 0.003 |  | EEF1A1 | 0.112 |  | GUSB | 0.18 |  | TBP | 0.928 | 0.447 | 1.766 |
 (2) | RPLP0 | 19 |  | GAPDH | 0.004 |  | GUSB | 0.112 |  | RPLP0 | 0.19 |  | RPL22 | 0.921 | 0.762 | 2.951 |
 (3) | RPL22 | 22 |  | RPLP0 | 0.007 |  | POLR2A | 0.123 |  | EEF1A1 | 0.19 |  | UBC | 0.916 | 0.660 | 2.360 |
 (4) | TBP | 22 |  | β-actin | 0.021 |  | β-actin | 0.124 |  | POLR2A | 0.24 |  | YWHAZ | 0.910 | 0.455 | 2.103 |
 (5) | β-actin | 22 |  | PPIB | 0.031 |  | TBP | 0.124 |  | RPL22 | 0.26 |  | β-actin | 0.905 | 0.537 | 3.093 |
 (6) | PPIB | 26 |  | RPL22 | 0.040 |  | RPLP0 | 0.125 |  | TUBB | 0.31 |  | GAPDH | 0.868 | 0.518 | 2.894 |
 (7) | POLR2A | 27 |  | UBC | 0.045 |  | PPIB | 0.206 |  | PPIB | 0.51 |  | PPIB | 0.739 | 0.446 | 2.094 |
 (8) | GAPDH | 30 |  | TBP | 0.050 |  | TUBB | 0.282 |  | TBP | 0.53 |  | RPLP0 | 0.440 | 0.126 | 0.818 |
 (9) | TUBB | 36 |  | POLR2A | 0.075 |  | RPL22 | 0.475 |  | β-actin | 0.66 |  | RPL | 0.355 | 0.250 | 0.996 |
 (10) | EEF1A1 | 38 |  | TUBB | 0.100 |  | RPL | 0.526 |  | RPL | 0.92 |  | GUSB | 0.328 | 0.138 | 0.561 |
 (11) | YWHAZ | 49 |  | EEF1A1 | 0.127 |  | GAPDH | 5.241 |  | GAPDH | 4.98 |  | POLR2A | 0.169 | 0.226 | 0.989 |
 (12) | UBC | - |  | YWHAZ | 0.850 |  | YWHAZ | 6.351 |  | YWHAZ | 5.99 |  | TUBB | 0.125 | 0.268 | 0.930 |
 (13) | RPL | - |  |  |  |  |  |  |  | UBC | 10.46 |  | EEF1A1 | -0.292 | 0.168 | 0.967 |
Direct cell–cell contact cultured of OCCM-30 and SV-PDL | ||||||||||||||||
 (1) | GUSB | 7 |  | POLR2A | 0.086 |  | GUSB | 0.005 |  | RPLP0 | 0.29 |  | POLR2A | 0.912 | 0.57 | 2.27 |
 (2) | POLR2A | 8 |  | GUSB | 0.089 |  | POLR2A | 0.007 |  | GUSB | 0.48 |  | GUSB | 0.889 | 0.62 | 2.36 |
 (3) | RPLP0 | 14 |  | RPL22 | 0.11 |  | RPL22 | 0.019 |  | PPIB | 0.48 |  | PPIB | 0.872 | 1.07 | 4.83 |
 (4) | RPL22 | 15 |  | RPLP0 | 0.212 |  | RPLP0 | 0.029 |  | POLR2A | 0.59 |  | RPL22 | 0.784 | 0.62 | 2.22 |
 (5) | PPIB | 16 |  | PPIB | 0.399 |  | PPIB | 0.058 |  | RPL22 | 1.09 |  | RPLP0 | 0.575 | 0.31 | 2.00 |
Co-cultured OCCM-30 control | ||||||||||||||||
 (1) | PPIB | 8 |  | PPIB | 0.211 |  | GUSB | 0.006 |  | RPLP0 | 0.31 |  | RPL22 | 0.986 | 1.5 | 5.31 |
 (2) | GUSB | 10 |  | POLR2A | 0.224 |  | PPIB | 0.013 |  | GUSB | 1.00 |  | PPIB | 0.977 | 0.95 | 4.34 |
 (3) | RPLP0 | 14 |  | RPLP0 | 0.325 |  | RPL22 | 0.028 |  | PPIB | 1.02 |  | GUSB | 0.977 | 0.94 | 3.46 |
 (4) | POLR2A | 14 |  | GUSB | 0.347 |  | POLR2A | 0.040 |  | POLR2A | 1.03 |  | POLR2A | 0.658 | 1.01 | 3.92 |
 (5) | RPL22 | 14 |  | RPL22 | 0.704 |  | RPLP0 | 0.045 |  | RPL22 | 1.59 |  | RPLP0 | 0.220 | 0.26 | 1.65 |
Co-cultured OCCM-30 with 3 indicated time (n = 18) | ||||||||||||||||
 (1) | PPIB | 9 |  | RPLP0 | 0.409 |  | PPIB | 0.016 |  | RPLP0 | 0.63 |  | PPIB | 0.949 | 0.72 | 3.25 |
 (2) | GUSB | 9 |  | GUSB | 0.478 |  | GUSB | 0.021 |  | POLR2A | 0.74 |  | GUSB | 0.767 | 0.6 | 2.18 |
 (3) | RPLP0 | 11 |  | PPIB | 0.515 |  | POLR2A | 0.027 |  | GUSB | 0.78 |  | RPL22 | 0.712 | 0.98 | 3.54 |
 (4) | POLR2A | 14 |  | POLR2A | 0.565 |  | RPL22 | 0.031 |  | PPIB | 0.78 |  | RPLP0 | 0.500 | 0.49 | 2.9 |
 (5) | RPL22 | 17 |  | RPL22 | 0.840 |  | RPLP0 | 0.035 |  | RPL22 | 1.27 |  | POLR2A | 0.427 | 0.64 | 2.48 |
Co-cultured SV-PDL control | ||||||||||||||||
 (1) | PPIB | 9 |  | RPLP0 | 0.308 |  | PPIB | 0.004 |  | RPLP0 | 0.11 |  | POLR2A | 0.998 | 0.65 | 2.59 |
 (2) | POLR2A | 11 |  | PPIB | 0.309 |  | RPL22 | 0.004 |  | PPIB | 0.52 |  | RPL22 | 0.983 | 0.68 | 2.59 |
 (3) | RPLP0 | 12 |  | POLR2A | 0.378 |  | POLR2A | 0.009 |  | RPL22 | 0.62 |  | GUSB | 0.980 | 0.53 | 1.85 |
 (4) | RPL22 | 12 |  | GUSB | 0.390 |  | GUSB | 0.011 |  | POLR2A | 0.74 |  | PPIB | 0.973 | 0.47 | 2.17 |
 (5) | GUSB | 16 |  | RPL22 | 0.732 |  | RPLP0 | 0.023 |  | GUSB | 0.78 |  | RPLP0 | 0.700 | 0.09 | 0.61 |
Co-cultured SV-PDL with 3 indicated time (n = 18) | ||||||||||||||||
 (1) | PPIB | 8 |  | POLR2A | 0.161 |  | PPIB | 0.005 |  | RPLP0 | 0.22 |  | PPIB | 0.980 | 0.31 | 1.44 |
 (2) | POLR2A | 10 |  | GUSB | 0.165 |  | RPLP0 | 0.015 |  | PPIB | 0.42 |  | POLR2A | 0.823 | 0.5 | 1.98 |
 (3) | RPLP0 | 11 |  | RPLP0 | 0.214 |  | GUSB | 0.016 |  | POLR2A | 0.60 |  | GUSB | 0.774 | 0.54 | 2.04 |
 (4) | GUSB | 12 |  | PPIB | 0.218 |  | POLR2A | 0.017 |  | GUSB | 0.60 |  | RPL22 | 0.746 | 0.57 | 2.02 |
 (5) | RPL22 | 19 |  | RPL22 | 0.422 |  | RPL22 | 0.019 |  | RPL22 | 0.74 |  | RPLP0 | 0.646 | 0.19 | 1.21 |