Fig. 3

ZINC08918027 (ZC) can inhibit both AURKA and AURKC. Prophase I-arrested mouse oocytes were treated with the indicated doses of ZC and matured in vitro to Metaphase I before freezing (A, B) or fixation (C). A Oocyte lysates (100 oocytes/per lane) were separated via SDS-PAGE and transferred to a membrane for western blotting to detect the activated forms of AURKA (pAURKA) and AURKC (pAURKC). Tubulin was used as a loading control. B Quantification of pAURKA and pAURKC normalized to Tubulin. Error bars show standard error of the mean. Data from three independent experiments (***p ≤ 0.001, (Unpaired Students t-Test, two-tailed)). C Wild-type (WT) or oocytes lacking both AURKB and AURKC were probed with anti-phosphorylated INCENP (pINCENP) antibodies (gray), α-tubulin (green) and mounted in DAPI (blue). Scale bar = 10 μm. D Quantification of integrated density of pINCENP from (C). WT DMSO n = 17, WT Zn 2 μM = 25, B cKO/C KO DMSO n = 27, B cKO/C KO Zn 2 μM = 33