Evaluation of multi-sample 16S ribosomal DNA sequencing for the diagnosis of postoperative bone and joint infections during antimicrobial treatment

Table 2 Detailed culture and sequencing results in episodes where multiple-sample sequencing added value in final determination of causative agents

Patient no	Type of infection		Biopsy 1	Biopsy 2	Biopsy 3	Biopsy 5	Biopsy 6	Biopsy 7
4	FRI	Sequencing	Klebsiella spp.	Streptocuccus australis	Ralstonias spp.	Alcalimonas spp.	Flavobacteriaceae	CoNS strain 1
4	FRI	Culture	CoNS strain 1	CoNS strain 2	Culture negative	Culture negative	Culture negative	CoNS strain 2
5	Postoperative arthritis^a	Sequencing	S. aureus	Not detected	Bacillus spp.
5	Postoperative arthritis^a	Culture	Culture negative	Culture negative	Culture negative
11	FRI	Sequencing	Burkholderia cepacia	Bacillus spp.
11	FRI	Culture	CoNS	CoNS
7	PJI	Sequencing	Bacilllus spp.	S. aureus + Bacillus spp.	S. aureus + Bacillus spp.	S. aureus + Bacillus spp.
7	PJI	Culture	Culture negative	Culture negative	Culture negative	Culture negative
25	FRI	Sequencing	Klebsiella spp.	Micrococcus luteus
25	FRI	Culture	Culture negative	Culture negative

S. aureus Staphylococcus aureus; Spp. Species; CoNs coagulase negative staphylococci; FRI fracture related infection; PJI prosthetic joint infection
^aAfter cruciate ligament reconstruction

ISSN: 1756-0500