Table 1 Details of the designed primers for the variant CYP2D6*10
Primers | Sequence (5′ to 3′) | Length (bp) | Product Size (bp) | Tm (°C) primer blast result | Tm (°C) |
|---|---|---|---|---|---|
CYP2D6*10 (rs1065852 C>T) | |||||
CYP2D6*10-CF | 5′TGGCAGCACAGTCAACACAGCAGGTTC 3′ | 27 | Control: 454 (T): 300 (C): 195 | 69.49 | 64.9 |
CYP2D6*10-CR | 5′CTGGTCCAGCCTGTGGTTTCACCCAC 3′ | 26 | 69.26 | 65.2 | |
CYP2D6*10-F(C) | 5ʹAACGCTGGGCTGCACGCTCCC 3′ | 21 | 68.11 | 67.7 | |
CYP2D6*10-R(T) | 5′GGCAGTGGCAGGGGGCCTGGGGA 3′ | 23 | 73.83 | 72.6 | |