Fig. 3

Effect of OA on DENV infection. BHK-21 cells were mock or DENV 2 infected before being treated with an equivalent volume of DMEM (DM), or DMSO solvent control (DS) or various concentrations of OA for 24 h. A After 24 h levels of DENV E, NS1, NS3 and NS5 were determined by western blot in parallel with host cell GAPDH as a control. B–E Band intensities from A were quantitated and normalized to expression of GAPDH. All experiments were undertaken independently in triplicate. Error bars show + S.E.M. *p ≤ 0.05, **p ≤ 0.01). Uncropped western blots for all replicates are provided in the associated supplemental materials