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Fig. 1 | BMC Research Notes

Fig. 1

From: Development of a quantitative methylation-specific droplet digital PCR assay for detecting Dickkopf-related protein 3

Fig. 1

Rational primer and probe design for ddMSP. A Graphical abstract of the detection system. B The prediction of CpG islands was performed using MethPrimer software. Cytosine bases that can be methylated are indicated in red, and the CpG island is presented in the blue area. C Sequences of the primer pairs and probes used in each assay, amplicon size, and the genome position. ccfDNA circulating cell-free DNA; ddMPCR droplet digital methylation-specific polymerase chain reaction

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