Fig. 1
From: Eip74EF is a dominant modifier for ALS-FTD-linked VCPR152H phenotypes in the Drosophila eye model
Eip74EF siRNA significantly rescues degenerative phenotypes while miR-34 overexpression results in severe degeneration or lethality: (A) Eip74EF siRNA expression (Eip74EF KD) significantly reduced dVCPR152H-induced abnormal eye phenotypes compared to mCherry siRNA expression (Control KD). miR-34 overexpression (OE) with dVCPR152H resulted in severe eye degeneration or lethality. miR-34 sponge with dVCPR152H resulted in slight enhancement in males (C) but did not show any effect in females (D). (B) Expression of transgenes with GMR-GAL4 to determine the lethality of the lines on their own. miR-34 OE lines were determined to be lethal, but mCherry siRNA, Eip74EF siRNA, and miR-34 sponge expression did not cause any effect on eyes. (C) Severity score quantification of male Drosophila. The raw data distribution is shown (upper panel) and the mean differences between control and experimental groups are plotted (lower panel) as dots with the vertical error bars indicating 95% confidence intervals. An independent statistical analysis was performed by One-way ANOVA with post-hoc Dunnett’s test (Control vs. Eip74EF siRNA: p < 0.0001, Control vs. miR-34 sponge: p = 0.037. Please note that there is no quantification for miR-34 OE males because there was N = 2, which was not enough for statistical analysis. (D) Severity score quantification of female Drosophila. One-way ANOVA with post-hoc Dunnett’s test (Control vs. Eip74EF siRNA: p < 0.0001, Control vs. miR-34 OE: p = 0.029 and 0.013, Control vs. miR-34 sponge: p = 1). Bloomington Stock Center lines from left to right: 35785, 29353, 63790 (miR-34 OE), 41158 (miR-34 OE.1), 61386