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Table 1 Whole Genome Sequencing Performance on s$v1.5 Illumina NovaSeq 6000 2 × 150 bp

From: Construction of high coverage whole-genome sequencing libraries from single colon crypts without DNA extraction or whole-genome amplification

 

PF Clusters

Paired-End Reads (B)

Yield (Gb)

%>Q30

Mean Quality

Run 6

12,913,455,779

26

3,489

90.8

35.4

Run 5

10,882,379,236

22

3,286

91.8

35.6

Run 4

12,811,554,090

26

3,869

91.0

35.4

Run 3

13,106,143,219

26

3,968

92.3

35.7

Run 2

12,401,208,646

25

3,745

91.8

35.6

Run 1

12,262,229,436

25

3,773

91.7

35.5

Average

12,396,161,734

25

3,688

91.6

35.5

Illumina Recommendations

16–20

2,400-3,000

> 85%